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Found 2 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
|
| Upstream tips |
Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
| Protocol tips |
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
| Upstream tips |
Protocol tips |
Downstream tips |
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment.
-Avoid repeated freeze-thaw cycles.
-The kit may be stored at 4°C for up to 6 months.
-Bring all reagents and samples to room temperature (18 - 25ºC) before use. |
-Levels of Dkk-1 may vary between different samples.
Optimal dilution factors
for each sample must be determined by the investigator.
-A standard curve must be run with each assay.
-Keep substrate solution protected from light.
-Read immediately after adding STOP solution. |
-Store your standard at <-70ºC after reconstitution, others at 4ºC. |
| Upstream tips |
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment.
-Avoid repeated freeze-thaw cycles.
-The kit may be stored at 4°C for up to 6 months.
-Bring all reagents and samples to room temperature (18 - 25ºC) before use. |
| Protocol tips |
-Levels of Dkk-1 may vary between different samples.
Optimal dilution factors
for each sample must be determined by the investigator.
-A standard curve must be run with each assay.
-Keep substrate solution protected from light.
-Read immediately after adding STOP solution. |
| Downstream tips |
| -Store your standard at <-70ºC after reconstitution, others at 4ºC. |
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