Live / Dead assay mammalian cells - C2C12

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 2 matching solutions for this experiment

Protocol tips
Add ethidium-calcein mixture, and incubate for 30-45 min at RT.

A shorter incubation time may be used if the dye concentrations or incubation temperature are increased.
Protocol tips
Add reagent and mix contents for 2 minutes to induce cell lysis.

Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal.
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