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Found 4 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
Prepare a staining solution of 2 uM calcein AM/4 uM EthD-III by adding 5 uL of 4 mM calcein
AM and 20 uL of 2 mM EthD-III to 10 mL of PBS or other serum-free buffer or medium |
Wash cells before adding calcein AM/EthD-III staining
solution to cover the cell monolayer.
Incubate the cells for 30-45 minutes at room temperature.
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| Upstream tips |
Prepare a staining solution of 2 uM calcein AM/4 uM EthD-III by adding 5 uL of 4 mM calcein
AM and 20 uL of 2 mM EthD-III to 10 mL of PBS or other serum-free buffer or medium |
| Protocol tips |
Wash cells before adding calcein AM/EthD-III staining
solution to cover the cell monolayer.
Incubate the cells for 30-45 minutes at room temperature.
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| Upstream tips |
Protocol tips |
Downstream tips |
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Add 4 μM of calcein-acetomethoxy (calcein AM-5 μL) and 2 mM of ethidium-bromide (20 μL).
Incubate this mixture for 30–45 min at room temperature in the dark |
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| Protocol tips |
Add 4 μM of calcein-acetomethoxy (calcein AM-5 μL) and 2 mM of ethidium-bromide (20 μL).
Incubate this mixture for 30–45 min at room temperature in the dark |
| Upstream tips |
Protocol tips |
Downstream tips |
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Add 8 µM ethidium homodimer and 2 µM calcein acetoxymethyl in PBS and incubate for 30~45 min at room temperature |
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| Protocol tips |
| Add 8 µM ethidium homodimer and 2 µM calcein acetoxymethyl in PBS and incubate for 30~45 min at room temperature |
| Upstream tips |
Protocol tips |
Downstream tips |
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Add 100 µl of assay solution to cells and incubate the mixture at 36.5 °C in 5 % CO2 atmosphere for 15 min. |
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| Protocol tips |
| Add 100 µl of assay solution to cells and incubate the mixture at 36.5 °C in 5 % CO2 atmosphere for 15 min. |
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