Mammalian cell culture media A2780

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Protocol tips
DMEM medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA). A2780/DPP was alternately fed with medium containing 9 μg/mL cisplatin to maintain drug-resistance and further cultured in drug-free medium for one week before follow-up experiments.
RPMI 1640

Nacalai Tesque

Protocol tips
RPMI 1640 medium containing 10% FCS, 1% penicillin/streptomycin (PAN Biotech, Aidenbach, Germany) and 1.5% L-glutamine.

Thermo Fisher Scientific

Protocol tips
DMEM/F12 containing 10% FBS.
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