Mammalian cell culture media Bovine Brain Microvascular Endothelial Cells: BBMVEC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 1 matching solution for this experiment

Upstream tips
Cells were transfected 4 hours prior to drug treatments and drug treatments were incubated for 24 h prior to hypoxic exposure in standard cell culture conditions (21% O2) then moved into hypoxic conditions (3% O2) for 24h.
Protocol tips
Cell cultures were placed in a special humidified hypoxic chamber for up to 48 h
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