Mammalian cell culture media Human Carotid Artery Smooth Muscle Cells: HCtASMC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Protocol tips
Experimental details for studies in primary human carotid artery smooth muscle cells (SMCs) and endothelial cells (HCtASMCs and human carotid artery endothelial cells; Cell Applications), using the Streamer apparatus (Flexcell International Corporation, Burlington, VT) with adjustable flow and shear stress conditions are described in the Methods in the Online Data Supplement.
Protocol tips
Cells were cultured up to passages 5 through 8 in subtype-specific growth medium (Cell Applications). Cells were seeded on 6-, 12-, or 24-well plates and treated or radiated at a density of 70% to 80%. Endothelial cells were cultured and seeded in gelatin-coated (Attachment Factor Protein 1X, Thermo Fisher Scientific) flasks and plates.
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