Mammalian cell culture media Human Epidermal Melanocytes: HEM

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Human Melanocyte Media

Cell Applications Inc

Protocol tips
Human epidermal melanocytes (HEM) were obtained from Cell Applications, Inc. (San Diego, CA, USA) (Cat. No. 104K-05a), maintained in melanocyte growth medium (Cell Applications, Inc.), incubated at 37°C in a 5% CO2 humidified incubator, and expanded for at least five passages. The medium was changed twice a week and subcultured when it was about 80% confluent.
Medium 254CF, Kit

Thermo Fisher Scientific

Protocol tips
supplement M-254 medium with HMGS-2, penicillin (100 U/ml), gentamicin (10 µg/ml) and amphotericin B (0.25 µg/ml) and culture the cells at 37°C in 5% CO2.
Protocol tips
melanocyte growth media (MGM) supplemented with melanocyte growth factors (MGF) (Lonza Walkersville Inc, Walkersville, MD)
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