Mammalian cell culture media MDCK

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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MEM (Eagle) Liquid Medium

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	MDCK II cells were maintained in Earle’s minimum essential medium supplemented with 4 mM glutamine, 0.2 mg/mL of both penicillin and streptomycin (Biochrom, Berlin, Germany), 10% (v/v) fetal calf serum (PAA, Pasching, Austria) in a 5% CO2 humidified incubator (HERA cell 150, Heraeus, Germany). Cells were subcultured weekly after reaching confluence by washing with PBS, followed by trypsinization and centrifugation at 110g.

Protocol tips
MDCK II cells were maintained in Earle’s minimum essential medium supplemented with 4 mM glutamine, 0.2 mg/mL of both penicillin and streptomycin (Biochrom, Berlin, Germany), 10% (v/v) fetal calf serum (PAA, Pasching, Austria) in a 5% CO2 humidified incubator (HERA cell 150, Heraeus, Germany). Cells were subcultured weekly after reaching confluence by washing with PBS, followed by trypsinization and centrifugation at 110g.

Protocol tips

MDCK II cells were maintained in Earle’s minimum essential medium supplemented with 4 mM glutamine, 0.2 mg/mL of both penicillin and streptomycin (Biochrom, Berlin, Germany), 10% (v/v) fetal calf serum (PAA, Pasching, Austria) in a 5% CO2 humidified incubator (HERA cell 150, Heraeus, Germany). Cells were subcultured weekly after reaching confluence by washing with PBS, followed by trypsinization and centrifugation at 110g.

Manufacturer protocol Publication protocol 1 Relevant paper

Dulbecco’s Modified Eagle’s Medium - high glucose

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Cells were propagated in Dulbecco's modified Eagle's medium (DMEM (Sigma D6429)), supplemented with 10% (v/v) heat‐inactivated foetal calf serum (FCS), penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37°C with 5% (v/v) CO2. G418 sulphate (Geneticin; GIBCO) (1 mg/mL) was added to the culture medium for propagating MDCK‐SIAT1 cells.

Protocol tips
Cells were propagated in Dulbecco's modified Eagle's medium (DMEM (Sigma D6429)), supplemented with 10% (v/v) heat‐inactivated foetal calf serum (FCS), penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37°C with 5% (v/v) CO2. G418 sulphate (Geneticin; GIBCO) (1 mg/mL) was added to the culture medium for propagating MDCK‐SIAT1 cells.

Manufacturer protocol Publication protocol 1 Relevant paper

Minimum Essential Media (MEM)

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	enriched with fetal bovine serum at 1%, 25 µg/L of gentamicin, and 200 mmol/L of L-glutamine (maintenance medium)

Protocol tips
enriched with fetal bovine serum at 1%, 25 µg/L of gentamicin, and 200 mmol/L of L-glutamine (maintenance medium)

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM–Dulbecco's Modified Eagle Medium

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal calf serum (FBS; Invitrogen) and 1% antibiotic/antimycotic (AA, GIBCO).

Protocol tips
supplemented with 10% fetal calf serum (FBS; Invitrogen) and 1% antibiotic/antimycotic (AA, GIBCO).

Manufacturer protocol Publication protocol 1 Relevant paper

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