Microarray Gene expression arrays - A-375 human melanoma Digoxigenin-11-dUTP

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

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Upstream tips
Avoid touching the surface of
the glass with your fingers. Skin oils and other substances, such as
lotions or ink, can fluoresce. If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.
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