Found 1 discussion for this experiment
1 year ago
1 year ago by Hollie Fowler
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
Found 1 matching solution for this experiment
|- Before adding CelLytic B reagent resuspend bacterial pellets in 1.0 ml of 50 mM Tris-HCl, pH 9.5 (three times).
- The pellets are lysed by shaking at 37°C for 30 minutes in 1.0 ml of CelLytic B reagent in 50 mM Tris-HCl, pH 9.5.
- The pellets are recovered by centrifugation and resuspended in 1.0 ml of 50 mM Tris-HCl, pH 9.5 (three times). Finally the pellets are resuspended in 1.0 ml of 50 mM Tris-HCl, pH 9.5.