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4 years ago
4 years ago by Hollie Fowler
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
Found 2 matching solutions for this experiment
|- Cells are harvested by centrifugation (5 min, 15,000 × g, 4 °C).|
|- Cell pellets are resuspended in 850 μl of lysis buffer (90 mm Tris, pH 8.0, and 100 μm fluorocitrate), and bacteria are mechanically disrupted in a Fast Prep instrument at a speed of 6.0 for 30 s. Lysates are centrifuged (1 min at 15,000 × g and 4 °C).|
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