Found 1 discussion for this experiment
2 years ago
2 years ago by Hollie Fowler
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
Found 2 matching solutions for this experiment
|- B-PER treatment did not solubilize the cells, instead it permeabilized the cells.|
|- Cells are resuspended in one-tenth of the original volume in the B-PER Bacterial Protein Extraction Reagent (Thermo Scientific, U.S.) containing EDTA-free protease inhibitor (Roche, Switzerland) and are shaken gently for 10 min in ice before centrifugation at 5000g for 10 min at 4 °C.|