Protein Ladder Unstained

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

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Found 15 matching solutions for this experiment

Upstream tips
• The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa.
Protocol tips
• The ladder is supplied in the gel loading buffer and is ready to use.
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 2.5 μl per well for general Western transferring.
Upstream tips
• Molecular Weight ranges from 20 to 150K
Protocol tips
Detection System & Loading Volume (μL)
• Colorimetric: 10
• Fluorescent: 5-10
• Chemiluminescence: 1-10
• ECL Substrate: 5-10
• SuperSignal West Pico Substrate: 5-10
• SuperSignal West Dura Substrate: 2-5
• SuperSignal West Femto Substrate: 1-5
Upstream tips
• Molecular Weight ranges from 20 to 150K
Protocol tips
Detection System & Loading Volume (μL)
• Colorimetric: 10
• Fluorescent: 5-10
• Chemiluminescence: 1-10
• ECL Substrate: 5-10
• SuperSignal West Pico Substrate: 5-10
• SuperSignal West Dura Substrate: 2-5
• SuperSignal West Femto Substrate: 1-5
Upstream tips
• Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins ranging from 10 to 200 kDa
Protocol tips
• The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Upstream tips
• Unstained Broad Range Protein Ladder is a mixture of 11 proteins ranging from 5 to 250 kDa.
Protocol tips
• The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Upstream tips
• Consisting of six polypeptides with molecular masses ranging from ~1.4 to ~26.6 kD.
Protocol tips
• Natural SDS-PAGE Standards are available in broad, low, high, and polypeptide molecular weight ranges, allowing calibration in almost any percentage gel.
Upstream tips
• Low Range Ladder is a mixture of eight purified proteins and peptides from 3.4 to 100 kDa
Protocol tips
• The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Upstream tips
• Recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa
Protocol tips
• Recommended load: 5 ul for clear visualization during electrophoresis on 15-well or 10-well mini-gel and for
Western transferring.
Upstream tips
• Size range—8 proteins spanning 60 to 250kDa
Protocol tips
• The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.
Upstream tips
• Unstained protein ladder ranges from (10 - 200 kDa)
Protocol tips
• Recommended load: 5 µL per well
Downstream tips
• The bands will become visible only once you stain your gel with a generic method, such as Coomassie blue.
BenchMark™ Protein Ladder

Thermo Fisher Scientific

Upstream tips
• BenchMark Protein Ladder consists of 15 unstained recombinant proteins ranging in molecular weight from 10 to 220 kDa.
Protocol tips
Recommended Load:
• Mini gel: 5 µL
• Midi gel: 10 µL
Upstream tips
• Thermo Scientific Unstained Protein Molecular Weight Marker is a mixture of seven native proteins ranging from 14.4 to 116 kDa
Upstream tips
• Unstained Protein Ladder ranges from 10 - 200 kDa.
Protocol tips
• Recommended load: 5 ul. for WB & SDS-PAGE.
Upstream tips
• Molecular weight ranges from 10-200 kDa
Protocol tips
• Load 3 μl directly onto a mini-gel.
Unstained Protein Standards

Bio-Rad Laboratories

Upstream tips
• Molecular masses range from 10 to 250 kD.
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