Reporter gene assay β-galactosidase substrates - CHO-K1

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 3 matching solutions for this experiment

Upstream tips
Seed 1 × 10^5 cells per well
Protocol tips
Add 150µl of Assay 2X Buffer to samples.

Incubate the reactions at 37°C for 30 minutes or until a faint yellow color has developed
Downstream tips
Read the absorbance at 420nm
Upstream tips
Fix cells after 72 hrs of miR-7 transfection.
Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
Protocol tips
Dilute Galacton-Plus substrate 1:100 with reaction buffer diluent to make a reaction buffer.

Add 70 ul of this buffer and incubate for 30-60 min
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