Reporter gene assay β-galactosidase substrates - COS-7

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 1 matching solution for this experiment

Upstream tips
Seed 4.8×10^5 cells
Protocol tips
Incubate for 90 min at 37 °C in 5% CO2.
Downstream tips
Read absorbance at 420 nm
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