Reporter gene assay β-galactosidase substrates - Huh7

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 2 matching solutions for this experiment

Protocol tips
Fix the cells with 0.5 ml of Fixative Solution for 10 - 15 min at room temperature.

Add 0.5 ml of the Staining Solution Mix to each well. Cover the plate. Incubate overnight at 37°C.
Protocol tips
Fix cells with Fixative Solution for 15 minutes at room temperature,

Incubate overnight (cells) at 37 ° C without Co2 and 48 hours (frozen sections)
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