Reporter gene assay β-galactosidase substrates - PANC-1

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 3 matching solutions for this experiment

β-Gal Assay Kit

Thermo Fisher Scientific

Protocol tips
Assay cells after 24h transfection.

Add 70 µl of ONPG and 200 µl 1X Cleavage Buffer with β-mercaptoethanol.

Incubate samples at 37°C for 30 minutes
Downstream tips
Read the absorbance at 420 nm
Protocol tips
Homogenize with reporter lysis buffer and centrifuged for 10 min at 12,000 g.

Add β-Glo assay solution and incubate for 1 h at room temperature.
Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
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