Reporter gene assay β-galactosidase substrates - SH-SY5Y

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 2 matching solutions for this experiment

Upstream tips
Pretreat cells with with or without H2S for 30 min and then stimulate with 50 μM H2O2 for 1 h.

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
Upstream tips
Culture cells for 48 h
Protocol tips
Wash cells and add β-Gal Reporter Gene Assay, incubate for 30 min at RT.

Add substrate reagent and incubate for 15-60 min at RT
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