Reporter gene assay β-lactamase substrates - HeLa

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 1 matching solution for this experiment

GeneBLAzer In Vivo Detection Kit

Thermo Fisher Scientific

Protocol tips
Add CCF2-AM substrate and incubate at 24 hpi for 30 minutes
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