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1 year ago
1 year ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
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|Total RNA from C6/36 cells at rest was isolated with RNAeasy (Qiagen). Total RNA from cells at rest was enriched for messenger RNA with oligo dT dynabeads (Invitrogen, Carlsbad, CA, USA). Total RNA from mock-infected and Zika-infected samples was subjected to SISPA multiplex library construction  and sequenced by Illumina NextSeq 1 × 150-bp sequencing.|
|Detached cells were transferred into a sterile 2 ml tube and were stored immediately at −80°C until total RNA extraction. Cells were homogenized in 350 µl RLT buffer in QIAshredder spin columns (Qiagen, Hilden, Germany) prior to total RNA extraction with Qiagen RNeasy Protect cell mini kit (Qiagen) according to manufacturer's instructions.|