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1 year ago
1 year ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
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|Total RNA was isolated from HEK293 cells and 2BS cells using an RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. The quality of the RNA samples was examined by quantifying the A260:A280 ratio (the minimal acceptable ratio is 1.7) and the 28S/18S by visualizing rRNA bands in agarose gel (the minimal acceptable ratio is 1.5).|
|After pre-incubation with or without MAPK inhibitors followed by stimulation with or without 10 µM HA (as indicated) for 4 h, the medium was removed and the cells were washed twice with PBS. Total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's protocol.|