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1 year ago
1 year ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
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|Total RNA from cultured healthy ESCs and corresponding rhCXCL12-treated healthy ESCs (n=18) was extracted using Qiagen miRNeasy Mini kit (Qiagen GmbH, Hilden, Germany). The quality of the extracted RNA was verified via absorbance measurements at wavelengths of 230, 260 and 280 nm using a spectrophotometer (NanoDrop 2000; Thermo Fisher Scientific, Inc.).|
|Total RNA was extracted using an AllPrep Micro Kit (Qiagen, Hilden, Germany). Total RNA samples were subjected to gene expression microarray analysis using a SurePrint G3 Human GE Microarray 8x60K Kit (product no. G4851A, design ID 28004, Agilent Technologies, Santa Clara, CA, USA) by following the manufacturer’s instructions.|