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|100 ng–50 μg total RNA, we recommend using the DynabeadsTM mRNA DIRECTTM Micro Kit Micro Kit (Cat. No. 61021).
50–400 μg total RNA, we recommend performing two rounds of oligo(dT) selection of the poly(A) RNA using the MicroPoly(A)PuristTM Kit (Cat. No. AM1919). Also, confirm the absence of 18S and 28S rRNA; for example, check the profile of the poly(A) RNA on an AgilentTM 2100 BioanalyzerTM instrument.