ROS assay cell type - C2C12

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Upstream tips
- 25000 cells per well were platted in 96 well plates in DMEM without phenol red
Protocol tips
- After 24h cells were treated with 25 μM DCFDA and incubated for 45 min at 37°C.

- For 10 min to 1 h they were treated with 0, 500 or 1000 μM H2O2.
Protocol tips
- Wells were loaded with Amplex red and exposed to CTX.

- For detection of cellular oxidants, cells loaded with dihydroethidium were exposed to CTX for 1 h and assayed.
Protocol tips
- Cells were incubated with 1 mM H2O2 for 30 min prior to lysis.
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