ROS assay cell type - Capan-2, PANC-1 pancreatic carcinoma

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

Fluoro H202

Cell Technology

Upstream tips
-0.5 × 10^6 cells were platted in 6 well plate.

- Cells were pretread with BITC at different concentration and time intervals
Protocol tips
- Cells were treated with 5 μM 6-carboxy-2′,7′-dichlorodihydrofluoroscein diacetate at 37°C for 15 min
Protocol tips
The generation of ROS was evaluated by measuring the levels of hydrogen peroxide produced in the cells using Fluorescent Hydrogen Peroxide/Peroxidase Detection Kit (Cell Technology) according to user's manual with slight modification.
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