ROS assay cell type - DU145

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Protocol tips
- After 24h of cell seeding, cells were treated with ROS detection solution and incubate under normal culture conditions for 1 h.

- After washing cells off from reagent, cells were treated with the solvent or Alternol in the presence or absence of the anti-oxidant
Protocol tips
- Cells were incubated with DCFH-DA for 1 h at 37°C.

- After incubation with DCFH-DA cells were treated with Tanshinone I and/or TRAIL for 1 h at 37°C
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