ROS assay cell type - H9c2 rat cardiomyocytes

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 4 matching solutions for this experiment

Upstream tips
- Cells were pretreated with Sal A for 4 h and then washed before adding 10 µM ATO and incubating for 24 h
Protocol tips
- Cells were resuspended in 400 mL of the 25 mM carboxy-H2DCFDA working solution, incubated in the dark for 20–30 min at 37 °C,
Protocol tips
- Cells were treated with DCFDA.

- After diffusion into the cells, DCFDA was deacetylated by cellular esterases to a non-fluorescent compound, which was later oxidized by ROS into 2′,7′-dichlorofluorescin (DCF)
Upstream tips
- 50,000 cells/well were seeded
Protocol tips
- Cells werre treated with α-TOS, NTα-TOS-NPs, or T-α-TOS-NPs for 12 h at 37 °C in 5% CO2.

- RIPA cell lysis buffer was
added and mixed for 2 min
Protocol tips
- Cells were treated with Superoxide Detection Solution and then incubated for 30 min
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