ROS assay cell type - HeLa

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Upstream tips
- It is recommended to keep the final concentration of solvents such as DMSO to ≤1%
Downstream tips
- Lower signals than expected when H2 O2 is applied to cells is due to catalase and glutathione peroxidase, that can rapidly eliminate H2O2
Upstream tips
- To reconstitute Oxidative stress detection reagent it was added to anhydrous dimethylformamide
Protocol tips
- Cells were incubated for for 1 h at 37 °C after adding detection reagent in complete media
Protocol tips
- Cells were incubated for 1h after adding fluorogenic probe.

- The assay was terminated by adding cell lysis buffer and incubating for 5 min.
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