ROS assay cell type - PC12

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Upstream tips
- Cells were seeded for 24h and then treated with 1X DCFH-DA and incubated for 60 min at 37 °C
Protocol tips
- Cells were treated with Ramizol™ at 0.1 mg mL−1, A-T (10 μM) and stressors 1 mM H2O2, 500 μM menadione and 100 mM glutamate for 60min
Protocol tips
- Cells were treated with 5 µM CM-H2DCFDA.

- Cells were incubated at 37C for 20 min.
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