siRNA / miRNA gene silencing Human - Melanoma cells (501 Mel and SK Mel 28) FANCD2 Polymer / Lipid

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Found 1 matching solution for this experiment

INTERFERin®

Polyplus transfections

Protocol tips
siRNA concentration- 20 nmol/L

siRNA SEQUENCE- GGAGAUUGAUGGUCUACUA-3′ and 5′-AACAGCCAUGGAUACACUUGATT-3′

Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization.

Remove culture medium from cells and add incubate mixture.

Incubate the plate at 37°C for 24 to 72 h.
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