siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) SIRT1

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

1 Matching solution
Start a discussion

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 1 matching solution for this experiment

SIRT1 siRNA and shRNA Plasmids (h)

Santa Cruz Biotechnology

Upstream tips	Protocol tips	Downstream tips
	Human PC-3 cells were provided by Antonio Filippini (Sapienza University), Cells were cultured in RPMI 1640 medium (Euroclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% antibiotics (Euroclone). For siRNA experiments, PC-3 cells were transfected with 40 pmol of human Sirt1 or with control siRNA purchased from Santa Cruz Biotechnology diluted in Opti-MEM (Gibco), using Lipofectamine RNAiMax as indicated by the manufacturer’s instructions. PC-3 cells were incubated for 48 h and then infected with VSVΔM51 at the indicated MOI.

Protocol tips
Human PC-3 cells were provided by Antonio Filippini (Sapienza University), Cells were cultured in RPMI 1640 medium (Euroclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% antibiotics (Euroclone). For siRNA experiments, PC-3 cells were transfected with 40 pmol of human Sirt1 or with control siRNA purchased from Santa Cruz Biotechnology diluted in Opti-MEM (Gibco), using Lipofectamine RNAiMax as indicated by the manufacturer’s instructions. PC-3 cells were incubated for 48 h and then infected with VSVΔM51 at the indicated MOI.

Protocol tips

Human PC-3 cells were provided by Antonio Filippini (Sapienza University), Cells were cultured in RPMI 1640 medium (Euroclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% antibiotics (Euroclone). For siRNA experiments, PC-3 cells were transfected with 40 pmol of human Sirt1 or with control siRNA purchased from Santa Cruz Biotechnology diluted in Opti-MEM (Gibco), using Lipofectamine RNAiMax as indicated by the manufacturer’s instructions. PC-3 cells were incubated for 48 h and then infected with VSVΔM51 at the indicated MOI.

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment