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Found 1 matching solution for this experiment
Cxcr4 siRNA + Lipofectamine® RNAiMAX Transfection Reagent
|Final concentartion of siRNA- 100 pmol per plate.
Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells.
Treat cells with Sdf-1 (10 ng/μl) after 24 h.
Incubate cells for another 48h at 37°C and quantify.
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