siRNA / RNAi /miRNA transfection Rat - AR42J Lipid based

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

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Found 3 matching solutions for this experiment

Protocol tips
Add 1:1 ratio of DNA to diluted
Lipofectamine® 2000 Reagent and Incubate for 5 minutes at room temperature.

Incubate cells for 1–3 days at 37°C. Then analyze transfected cells.
Protocol tips
Add diluted siRNA to transfection reagent within 5 min.

Incubate for 15-20 minutes at room temperature and add to cells.
Protocol tips
Dilute siRNA in culture medium without serum.

Incubate the samples for 5–10 min at room temperature
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