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|Always add your plasmid to the buffer before adding Xfect Polymer.
dilute 5 µg of your plasmid DNA with Xfect Reaction Buffer to a final volume of 100 µl.
Incubate for 10 min at room temperature.
Add the nanoparticle complex solution dropwise to the cell culture medium.
Incubate the plate at 37°C for 4 hr to overnight.
| Seed ~5 × 10^4 cells
|Dilute 1µg of DNA in 100µL of serum-free DMEM or other serum-free growth medium.
Incubate 15-20 minutes at room temperature.
Add transfection reagent/DNA mixture to samples and incubate at 37°C in a CO2 incubator.
|Analyse expression after 24-48 hours|