siRNA / RNAi /miRNA transfection Rat - IEC Cationic lipid based

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

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Found 2 matching solutions for this experiment

siRNA Transfection Reagent

Santa Cruz Biotechnology

Upstream tips	Protocol tips	Downstream tips
	Incubate the mixed reagents for 30 min at room temperature prior to use. Incubate cells with siRNA transfection reagent mixture (solution A + solution B) for 6 h at 37°C in a CO2 incubator and remove to replace 1X normal growth medium for an additional 24 h.

Protocol tips
Incubate the mixed reagents for 30 min at room temperature prior to use. Incubate cells with siRNA transfection reagent mixture (solution A + solution B) for 6 h at 37°C in a CO2 incubator and remove to replace 1X normal growth medium for an additional 24 h.

Manufacturer protocol Publication protocol 1 Relevant paper

HiPerFect Transfection Reagent

Qiagen

Upstream tips	Protocol tips	Downstream tips
	Dilute siRNA in culture medium without serum. Incubate the samples for 5–10 min at room temperature

Protocol tips
Dilute siRNA in culture medium without serum. Incubate the samples for 5–10 min at room temperature

Manufacturer protocol Publication protocol 1 Relevant paper

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