Found 1 discussion for this experiment
1 year ago
1 year ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 2 matching solutions for this experiment
|Treat with Proteinase K Solution for 15-30 minutes or Cytonin for 30 minutes.
Treat with Labeling Reaction Mix and incubate for 60
minutes at 37˚C in a humidity chamber.
Treat with TACS Blue LabelTM (page 5), and incubate for 2-7
|If no staining in TACS Nuclease, Increase incubation time with Proteinase K (up to 60 minutes) or Cytonin™ (up to overnight at 4 oC).|