Found 1 discussion for this experiment
2 years ago
2 years ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 3 matching solutions for this experiment
|Fix sample in 4% formaldehyde for 1 h before freezing.|
|Fix sample in 4% formaldehyde for 5 min before using sample.
Block samples in 10% donkey serum in 0.1% Triton-X for 30 min at room temperature.
Incubate primary antibody overnight at 4 °C and with secondary antibodies for 1 h at room temperature
|Fix cell samples with Fixation solution for 1 h.
Incubate in Permeabilisation solution (0.1% Triton X-100 in PBS) for 2 min on ice.
Label and incubate for 60 min at +37°C in a humidified atmosphere in the dark
|If low labelling is found, reduce fixation time or try 2% buffered paraformaldehyde|