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2 years ago
2 years ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 3 matching solutions for this experiment
|- Cell were pretreated with 50 μM, 75 μM, and 100 μM of honokiol for 12, 24, 48, or 72 hours.|
|- Cells were fixed with 1% (w/v) paraformaldehyde in PBS and place on ice for 15 minutes|
|- If signal intensity is low increase the incubation time for the DNA-labeling reaction|
|- Cells were fixed with 4% paraformaldehyde for 20 min.
- Permeabilized with PBS containing 0.1% Triton X-100 and 0.1% glycine for 2 min on ice
|- When Nonspecific labelling is present, reduce concentration of TdT.
- When high background is present reduce concentration of labelling mix by 50%