DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

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MethylCap kit

Diagenode

Upstream tips	Protocol tips	Downstream tips
	Always thaw the MethylCap protein on ice. Preferably aliquots it to avoid multiple freeze-thaw cycles.

Protocol tips
Always thaw the MethylCap protein on ice. Preferably aliquots it to avoid multiple freeze-thaw cycles.

Manufacturer protocol Publication protocol 1 Relevant paper

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