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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - It is recommended to keep the final concentration of solvents such as DMSO to ≤1% |
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- Lower signals than expected when H2 O2 is applied to cells is due to catalase and glutathione peroxidase, that can rapidly eliminate H2O2 |
| Upstream tips |
| - It is recommended to keep the final concentration of solvents such as DMSO to ≤1% |
| Downstream tips |
| - Lower signals than expected when H2 O2 is applied to cells is due to catalase and glutathione peroxidase, that can rapidly eliminate H2O2 |
| Upstream tips |
Protocol tips |
Downstream tips |
| - To reconstitute Oxidative stress detection reagent it was added to anhydrous dimethylformamide |
- Cells were incubated for for 1 h at 37 °C after adding detection reagent in complete media |
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| Upstream tips |
| - To reconstitute Oxidative stress detection reagent it was added to anhydrous dimethylformamide |
| Protocol tips |
| - Cells were incubated for for 1 h at 37 °C after adding detection reagent in complete media |
| Upstream tips |
Protocol tips |
Downstream tips |
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- Cells were incubated for 1h after adding fluorogenic probe.
- The assay was terminated by adding cell lysis buffer and incubating for 5 min. |
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| Protocol tips |
- Cells were incubated for 1h after adding fluorogenic probe.
- The assay was terminated by adding cell lysis buffer and incubating for 5 min. |
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