siRNA / miRNA gene silencing Mouse - BV2 TIMP-2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Timp2 siRNA

Thermo Fisher Scientific

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
Seed 0.5 × 10^5 cells per well in a 12-well plate.	siRNA TIMP-2 -100 pM TIMP-2 siRNA #1 (5′-GAAGGAGUAUCUAAUUGCATT-3′), TIMP-2 siRNA #2 (5′-UCCACAGACUUCAGCGAAU-3′), Mix diluted RNA and diluted siPORT™ NeoFX™ Transfection Agent; incubate 10 min at room temp, Incubate the transfected cells at 37°C for 48h.

Upstream tips
Seed 0.5 × 10^5 cells per well in a 12-well plate.
Protocol tips
siRNA TIMP-2 -100 pM TIMP-2 siRNA #1 (5′-GAAGGAGUAUCUAAUUGCATT-3′), TIMP-2 siRNA #2 (5′-UCCACAGACUUCAGCGAAU-3′), Mix diluted RNA and diluted siPORT™ NeoFX™ Transfection Agent; incubate 10 min at room temp, Incubate the transfected cells at 37°C for 48h.

Manufacturer protocol Publication protocol 1 Relevant paper

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