Bacterial cell culture media Clostridium tetani

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

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Found 3 matching solutions for this experiment

Protocol tips
-Supplement the medium with Resazurine in anaerobic boxes at 35°C
-Final pH 7.1 ± 0.2
-Cultural characteristics after 48-72 hours at 35°C
THIOGLYCOLLATE BROTH USP

Thermo Fisher Scientific

Protocol tips
-PREPARE FRESHLY OR BOIL AND COOL THE MEDIUM JUST BEFORE USE.
-pH 7.1 ± 0.2 @ 25°C
Nutrient broth

Merck Millipore

Protocol tips
-pH: 7.0 ± 0.2 at 25 °C.
-Incubation: 24 h at 35 °C aerobically.
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