ORIS™ Cell Migration Assay

Protocol tips

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	Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 24 hours to permit cell attachment.

Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 24 hours to permit cell attachment.

Publication protocol

Cell migration assays were performed using Oris Migration Assay kits (Platypus Technologies, USA) as described previously [21]. Briefly, 5 × 104 cells were inoculated in each well of a 96-well plate in the presence of the stoppers. The central stopper was removed after 12 h of incubation to allow the cells to migrate into the central area. Photographs were obtained to document the unoccupied area at t = 0 h (as stopper removed). The cells that had migrated into the central area were then photographed at 32 h for TW01 and HONE-1 cells and at 48 h for FaDu cells. The area of the unoccupied central region of each well was determined by ImageJ (https://imagej.nih.gov/ij/), and the percentage of migration was calculated using the equation: % Migration = [(Areat = 0 — Areat = Δ) × Areat = 0] × 100%, where Δ = 32 or 48 h [22].

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Manufacturer protocol

Download the product protocol from Platypus Technologies for ORIS™ Cell Migration Assay below.

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