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Discussion

1 year ago

Keith L. Morrison

4

Votes

3

Comments

siRNA/RNAi/miRNA transfection human

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Experiment: siRNA / RNAi /miRNA transfection Human

Discussion

5 months ago

Natalia Penaranda

1

Vote

1

Comment

Best Antibody

which is a good monoclonal anibody for this protein?

Experiment: Western blotting GAPDH

Discussion

5 months ago

Alexya Azhakesan

1

Vote

0

Comments

Impact of using FBS

I work with Human liver organoids and I would like to know the impact of FBS on the organoids. Since the composition of FBS is unknown, would you recommend any alternatives such as human platelet lysate?

Experiment: 3D Cell Culture Media Human liver organoids

Discussion

5 months ago

Alexya Azhakesan

1

Vote

0

Comments

Impact of using FBS

I have tried to fabricate Liver organoids and would like to study the impact of FBS on healthy and tumor organoids. Since the compositions of FBS is unknown, do you recommend any alternatives like Human platelet lysate, etc?

Experiment: 3D Cell Culture Media Human liver organoids

Discussion

5 months ago

Christos Karampelas

1

Vote

0

Comments

Is a bacterial nuclease contamination possible during protein purification?

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Experiment: Protein isolation Bacteria - Escherichia coli

Discussion

5 months ago

Hari Krishn Thulluru

2

Votes

0

Comments

gemonic DNA elimination

Can i use genomic eliminator colums

Experiment: RNA isolation / purification Cells - immortalized C6

Discussion

5 months ago

Shantha Devi Udayappan

2

Votes

0

Comments

is it suitable for human adipose tissue biopsy samples.

The kit works good in human tissue biopsy samples even with minimum amount of tissue.

Experiment: RNA isolation / purification Tissue - Rat Adipose

Discussion

5 months ago

Priyanka Majumder

1

Vote

0

Comments

Lipofectamin review

Worked fine for transfection . High efficiency.

Experiment: siRNA / RNAi /miRNA transfection Human - HeLa Lipofectamine

Discussion

5 months ago

Akshay Harapanahalli

0

Votes

0

Comments

Passage number

HeLa cells I used are passage 40. Are these cells good to perform Cell cycle analysis on?

Experiment: Cell cycle assay human - HeLa

Discussion

6 months ago

Stan Stan

0

Votes

0

Comments

Test

Test

Experiment: cDNA synthesis Tissue

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