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Discussion
2 weeks ago
Shorouq Alqazaqi
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Hello Iam a phd student in pharmacy and i want to know if this technology is suitable to knockout or silencing part of the gas5 gene in BV2 cells please
Experiment: siRNA / miRNA gene silencing Mouse - BV2 TLR4
Discussion
2 years ago
Natalia Penaranda
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which is a good monoclonal anibody for this protein?
Experiment: Western blotting GAPDH
Discussion
3 years ago
Keith L. Morrison
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I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?
Experiment: siRNA / RNAi /miRNA transfection Human Cells
Discussion
2 years ago
Alexya Azhakesan
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I work with Human liver organoids and I would like to know the impact of FBS on the organoids. Since the composition of FBS is unknown, would you recommend any alternatives such as human platelet lysate?
Experiment: 3D Cell Culture Media Human liver organoids
Discussion
2 years ago
Alexya Azhakesan
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I have tried to fabricate Liver organoids and would like to study the impact of FBS on healthy and tumor organoids. Since the compositions of FBS is unknown, do you recommend any alternatives like Human platelet lysate, etc?
Experiment: 3D Cell Culture Media Human liver organoids
Discussion
2 years ago
Christos Karampelas
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I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?
Experiment: Protein isolation Bacteria - Escherichia coli
Discussion
2 years ago
Hari Krishn Thulluru
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Can i use genomic eliminator colums
Experiment: RNA isolation / purification Cells - immortalized C6
Discussion
2 years ago
Shantha Devi Udayappan
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The kit works good in human tissue biopsy samples even with minimum amount of tissue.
Experiment: RNA isolation / purification Tissue - Rat Adipose
Discussion
2 years ago
Priyanka Majumder
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Worked fine for transfection . High efficiency.
Experiment: siRNA / RNAi /miRNA transfection Human Cells - HeLa Lipofectamine
Discussion
2 years ago
Akshay Harapanahalli
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HeLa cells I used are passage 40. Are these cells good to perform Cell cycle analysis on?
Experiment: Cell cycle assay human - HeLa