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Discussion
1 week ago
Stan
0
Votes
0
Comments
Discussion
1 year ago
Nicolai Brandt
3
Votes
3
Comments
What DNA isolation kit would work for insect samples?
Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?
Experiment: DNA isolation / purification Tissue
Discussion
3 weeks ago
Lionel Petitiaux (Efty)
1
Vote
1
Comment
Discussion
1 year ago
Eufrosina Sagese
4
Votes
6
Comments
Is a knockdown using shRNA permanent?
Is a knockdown using shRNA permanent and if not is there a known duration?
Experiment: shRNA gene silencing Human
Discussion
1 year ago
Keith L. Morrison
4
Votes
3
Comments
siRNA/RNAi/miRNA transfection human
I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?
Experiment: siRNA / RNAi /miRNA transfection Human
Discussion
1 year ago
Jana Fassbinder
3
Votes
1
Comment
What is the optimal concentration for primers in qPCR?
What is the optimal concentration for primers in qPCR? My total volume is 20μl per reaction.
Experiment: PCR Quantitative real-time PCR
Discussion
1 year ago
Paul G. Macon
2
Votes
4
Comments
Some help with RNA isolation using Trizol
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
Experiment: RNA isolation / purification Bacteria
Discussion
1 year ago
Sander J. Mikkelsen
2
Votes
2
Comments
endothelial cells vs HUVEC cells?
What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?
Experiment: Angiogenesis assay human
Discussion
1 year ago
S Janardhan
3
Votes
2
Comments
Reactivate dormant Lactobacillus Paracasei
Hello fellow Labettors, I would like to know what would be the best method/medium to reactivate my dormant Lactobacillus paracasei cells?. Any suggestions are greatly appreciated.
Experiment: Bacterial cell culture media Lactobacillus paracasei
Discussion
1 year ago
Mario Udinese
3
Votes
2
Comments
Floxing mice with CRISPR
Hi everyone! I am planning on floxing mice with CRISPR but I am having trouble deciding which region to target. Do you have any tips on choosing?
Experiment: CRISPR Mouse