QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 0.5 × 10^6 to 1 × 10^6 cells	Add 10% FBS complete medium in the lower chamber. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).	Read plate at 570nm

Upstream tips
Seed 0.5 × 10^6 to 1 × 10^6 cells
Protocol tips
Add 10% FBS complete medium in the lower chamber. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).
Downstream tips
Read plate at 570nm

Publication protocol

The invasion potential was assessed using Chemicon cell invasion assay kit (Millipore, Billerica, MA) as directed by the manufacturer. Transwells with 8 μM pores coated with Matrigel were used for the assay. Serum-starved (0.5 × 106 to 1 × 106) cells (12-16 hours) in 300 μl of 10% FBS complete medium were seeded in the upper chambers, while lower wells were filled with 500 μl of 10% FBS complete medium. Invading cells on the lower surface were fixed and stained. The stain was dissolved in 200 μl of 10% acetic acid and measured at 570 nm. Invasive potential is derived by normalizing with the readings from blank transwell inserts.

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