Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent). |
Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4- 6% CO2). |
|
Upstream tips |
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent). |
Protocol tips |
Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4- 6% CO2). |
Publication protocol
The cell invasion assay was performed with QCM 24-well Invasion Assay kit (Chemicon International). This cell invasion assay was performed in an invasion chamber, based on the Boyden chamber principle. This kit contains 24 inserts and each insert contains an 8-μm pore size polycarbonate membrane coated with a thin layer of ECM. The method was described previously [10].
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Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - LoVo using QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric from Merck Millipore.
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Identification and characterization of in stage II and III colorectal carcinoma
Manufacturer protocol
Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.
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