ROS-ID® Total ROS/Superoxide detection kit

Protocol tips

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	- After wash step, cells were stained with ROS detection solution and incubated at 30 min, 37˚C

Protocol tips
- After wash step, cells were stained with ROS detection solution and incubated at 30 min, 37˚C

Publication protocol

Seeding the cells on 6-well plates (Cornig Costar, Fisher Scientific) one day prior to the experiment was performed to assure 50-70% confluence. Staining for flowcitometric analysis was performed using total ROS Detection Kit ( Enzo Life Sciences). Briefly, following treatment, samples were suctioned to remove media and detached using sterile scraping technique. Collection of cells in 5 mL polystyrene tubes and staining (30 min, 37˚C) with the ROS detection solution provided by the manufacturer was performed. Reactive oxygen species accumulation was evaluated using a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA, USA) with green (FL1 channel-530 nm) The Total ROS Detection Kit (Enzo Life Sciences) was used for cell cultures staining following administration of MWCNTs-PEG and Laser irradiation. Next, suspensions of Panc-1 cells were analyzed by flow cytometry in accordance with the manufacturer's instructions.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

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