Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit

Angiogenesis assay human - HMVEC

Experiment
Angiogenesis assay human - HMVEC
Product
Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit from Trevigen
Manufacturer
Trevigen

Protocol tips

Protocol tips
- After seeding for 24h cells are treated with Galectin-3 for another 24h.

Publication protocol

HMVECs (1×105 cells/well) were cultured in 24-well plates for 24 hr before treatment with galectin-3 for 24 hr. The culture media was collected and used, with or without subsequent introduction of a combination of recombinant cytokines or a combination of neutralizing anti-cytokine antibodies, to assess migration of fresh HMVECs through basement matrix proteins using the In Vitro Angiogenesis Assay Endothelial Cell Invasion kit; or to assess HUVECs tubule formation using the In Vitro Angiogenesis Assay Endothelial Cell Tube Formation kit. The length and branch points of tubules formed were quantified using ImageJ (http://rsbweb.nih.gov/ij/).


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Discussion

Discussion

5 years ago

Author: Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Angiogenesis assay human - HMVEC using Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit from Trevigen.

Paper title
Increased circulation of galectin-3 in cancer induces secretion of metastasis-promoting cytokines from blood vascular endothelium
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Manufacturer protocol

Download the product protocol from Trevigen for Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit below.

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