Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation

Angiogenesis assay human - HMVEC

Experiment
Angiogenesis assay human - HMVEC
Product
Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation from Corning
Manufacturer
Corning

Protocol tips

Protocol tips
- The pretreated cells plus RA SF (1:20) or flagellin (10 ng/ml) were added to the polymerized matrigel and the plate was incubated for 16h at 37°C

Publication protocol

To perform the matrigel tube formation assay, BD Matrigel Matrix (BD BioCoat tube formation kit, 50 µl) was polymerized for 30 min at 37°C in a 96-well plate. In order to determine whether TLR5 ligation is pivotal for RA SF induced endothelial tube formation, HMVECs/HUVECs were incubated with antibody to TLR5 or IgG (10µg/ml) for 45 minutes at 37°C. The pretreated cells plus RA SF (1:20) or flagellin (10 ng/ml) were added to the polymerized matrigel and the plate was incubated for 16h at 37°C. Each condition was performed in triplicate, bFGF (20 ng/ml; R&D systems) was used as a positive control, and PBS was used as a negative control. Following incubation, the number of branch points/tubes was quantified as previously described (17, 19, 20).


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Discussion

Discussion

5 years ago

Author: Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Angiogenesis assay human - HMVEC using Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation from Corning.

Paper title
Angiogenesis in rheumatoid arthritis is directly fostered by TLR5 ligation and indirectly through IL-17 induction
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Manufacturer protocol

Download the product protocol from Corning for Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation below.

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