In Vitro Angiogenesis Assay Kit

Angiogenesis assay human - HAEC

Experiment
Angiogenesis assay human - HAEC
Product
In Vitro Angiogenesis Assay Kit from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
- Cells are treated with or without aspalatone (10 μM, 20 μM, 50 μM, and 100 μM) and incubated for additional 18 h.

Publication protocol

HAEC tube formation assay was performed by using an in vitro angiogenesis kit from EMD Millipore following manufacturer's instructions. Briefly, 50 μL of diluted ECM matrix solution was added to each well of 96-well plate and incubated at 37°C to allow the matrix to solidify. Growth arrested HAECs were harvested and then seeded carefully without disturbing the solidified matrix at 1 × 104 cells/well in the absence and presence of aspalatone (10 μM, 20 μM, 50 μM, and 100 μM) and incubated for additional 18 h. Photographs were taken and the length of the capillary network was quantitated as described earlier


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Discussion

Discussion

5 years ago

Author: Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Angiogenesis assay human - HAEC using In Vitro Angiogenesis Assay Kit from Merck Millipore.

Paper title
Aspalatone Prevents VEGF-Induced Lipid Peroxidation, Migration, Tube Formation, and Dysfunction of Human Aortic Endothelial Cells
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Manufacturer protocol

Download the product protocol from Merck Millipore for In Vitro Angiogenesis Assay Kit below.

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